Police are investigating an affray on the Westgate Freeway earlier this month.
Police have been told traffic had slowed in the outbound lanes of the Westgate just after the Williamstown Road exit about 4pm on February 11.
Two men in a grey BMW pulled up next to a charter bus, believed to be carrying a group of men returning to Geelong from a bucks day, police said.
An empty beer bottle was allegedly thrown from the bus and smashed into the bonnet of the car and dented the vehicle.
The driver of the car stopped the bus and he and his passenger got out and approached the bus driver.
A number of occupants got out of the bus and assaulted the victims before turning their attention to the car and smashing windows and mirrors before jumping on the car.
The group then got onto the bus which drove off, police said.
One victim suffered a dislocated shoulder while the other suffered facial injuries.
Anyone who witnessed the incident or with dashcam footage or CCTV is urged to contact Crime Stoppers on 1800 333 000 or www.crimestoppersvic.com.au.